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array integrated fluidic circuits chip  (fluidigm)


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    Structured Review

    fluidigm array integrated fluidic circuits chip
    Array Integrated Fluidic Circuits Chip, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96%2E96+dynamic+array+integrated+fluidic+circuit+chip/96%2E96+Dynamic+Array+IFC+for+Genotyping/pmc11002141-115-10-15
    Average 95 stars, based on 416 article reviews
    array integrated fluidic circuits chip - by Bioz Stars, 2026-09
    95/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Cholestasis alters brain lipid and bile acid composition and compromises motor function in neonatal piglets
    Article Snippet: For the remaining genes, cDNA was synthesized in triplicates from 500 ng total RNA using the QuantiTect Reverse Transcription Kit (Qiagen), pre‐amplified using the TaqMan PreAmp Master Mix (Applied Biosystems) and treated with exonuclease I (New England Biolabs) as previously described (Skovgaard et al., ). .. Primer sets and pre‐amplified and exonuclease‐treated cDNA including non‐template and non‐reverse transcriptase controls were loaded on a 96.96 Dynamic Array Integrated Fluidic Circuit chip (Fluidigm) and analyzed on the BioMark real‐time PCR system (Fluidigm; Skovgaard et al., ). .. C q values were obtained from the Fluidigm Real‐Time PCR Analysis software (version 4.7.1; Fluidigm) and processed in GeneEx (version 8; MultiD) by correcting for PCR efficacy, normalizing to validated reference genes ( ACTB , GAPDH , HPRT1 , RPL13A , YWHAE ; Table ) and averaging cDNA triplicates, as previously explained (Skovgaard et al., ).

    Positive Control:

    Article Title: Multi-transcript profiling in archival diagnostic prostate cancer needle biopsies to evaluate biomarkers in non-surgically treated men
    Article Snippet: Taqman primers (Applied Biosystems) with the shortest amplicons lengths for these genes were acquired for this study: E Cadherin: Hs01023894_m1, EGFR: Hs01076078_m1, EZH2: Hs00544833_m1, PTEN: Hs02621230_s1, MSMB: Hs00738230_m1, amp length 72 PSMA: Hs01020194_mH, AR: Hs00171172_m1, PCA3: Hs01371939_g1, B-actin: Hs01060665_g1, GAPDH: Hs03929097_s1, RPL13: Hs00742932_s1, amp length 81. .. The Fluidigm® 96.96 Dynamic Array integrated fluidic circuit chip was used to simultaneously profile the 9 gene panel (including β actin) in the 60 tumours as well as 2 benign prostate samples, 1 cancer line (PC3), 1 benign cell line (PNT2), a RNA positive control (Clontech, CA, USA) and a negative (water) control. .. Aliquots of each Gene Expression Assay were made up to a 10x concentration [2.5 μl of 20X Taqman Assay (Applied Biosystems) and 2.5 μl 2X Loading Reagent (Fluidigm®)].

    Control:

    Article Title: Multi-transcript profiling in archival diagnostic prostate cancer needle biopsies to evaluate biomarkers in non-surgically treated men
    Article Snippet: Taqman primers (Applied Biosystems) with the shortest amplicons lengths for these genes were acquired for this study: E Cadherin: Hs01023894_m1, EGFR: Hs01076078_m1, EZH2: Hs00544833_m1, PTEN: Hs02621230_s1, MSMB: Hs00738230_m1, amp length 72 PSMA: Hs01020194_mH, AR: Hs00171172_m1, PCA3: Hs01371939_g1, B-actin: Hs01060665_g1, GAPDH: Hs03929097_s1, RPL13: Hs00742932_s1, amp length 81. .. The Fluidigm® 96.96 Dynamic Array integrated fluidic circuit chip was used to simultaneously profile the 9 gene panel (including β actin) in the 60 tumours as well as 2 benign prostate samples, 1 cancer line (PC3), 1 benign cell line (PNT2), a RNA positive control (Clontech, CA, USA) and a negative (water) control. .. Aliquots of each Gene Expression Assay were made up to a 10x concentration [2.5 μl of 20X Taqman Assay (Applied Biosystems) and 2.5 μl 2X Loading Reagent (Fluidigm®)].



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